N/H/K-Ras Polyclonal Antibody

SKU: E-AB-32152

Size: 200μL

Storage: Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping: The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date: 12 months

Category ID_II: Primary Antibodies

Category ID_III: Polyclonal Antibodies

Abbreviation: N/H/K-Ras

Target Synonym: NRAS;HRAS1;GTPase NRas;Transforming protein N-Ras;HRAS;HRAS1;GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;KRAS;KRAS2;RASK2;GTPase KRas;K-Ras 2;Ki-Ras;c-K-ras;c-Ki-ras

Research Areas: Cancer;Epigenetics and Nuclear Signaling;Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species reactivity: Human;Mouse;Rat

Application: WB;IHC-p

Isotype: IgG

Clonality: Polyclonal

Clone NO.:

UNIProt ID: P01111,P01112,P01116

Accession:

Background: NRAS an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Concentration: 1 mg/mL

Immunogen: Synthesized peptide derived from the N-terminal region of human N/H/K-Ras

Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Purification method: Affinity purification

Dilution: WB 1:500-1:2000;IHC 1:100-1:300

Calculated MW: 21kDa

ObservedMW: 21kDa