Human Tumor Necrosis Factor α (TNF-α) ELISA Kit

SKU: AE13959HU

Size:96T. Other sizes are also available. Please Inquire.

Species Reactivity:Human (Homo sapiens)

UniProt:P01375

Abbreviation:TNF-α

Alternative Names:TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein|TNF superfamily; member 2|TNF; macrophage-derived|TNF; monocyte-derived|cachectin|tumor necrosis factor alpha; TNFSF7

Application:ELISA

Range:15.6-1000 pg/mL

Sensitivity:6.5 pg/mL

Intra-AssayCV:≤6.7%

Inter-AssayCV:≤11.6%

Recovery:1,04

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).