2x GB-AMP™ PaCeR™ HP™ Master Mix

SKU: PCR-002-01

2x GB-AMP™ PaCeR™ HP™ Master Mix is a convenient ready-to-use mixture of GB-AMP™ PaCeR™  HP™ DNA polymerase, dNTP, buffer and magnesium ion. To set up a PCR reaction, you will only need to add the primers, the template and water. 

 

For a brochure on this product, please follow this link. 

 

GB-AMP™ PaCeR™  HP™ DNA polymerase was derived from Pfu DNA Polymerase, after several iterative rounds of protein engineering, the enzyme boasts the on-board unique extension factor, specificity-promoting factors and plateau-inhibiting factor. It is a Fast Pfu-derived polymerase. 

• Super Fidelity: 53-fold higher than Taq DNA Polymerase and 6-fold better than Pfu DNA polymerase
• Long Range capability: amplify fragments as long as 40 kbλ DNA, 20 kb genomic DNA and 10 kb cDNA
• Difficult PCR suitability: Amplify targets with as high as 85% GC
• Direct-PCR: resists inhibitors in crude lysates of bacteria, fungi, whole blood, cultured cells, plant tissues, etc.
• Superior Hot-Start enzyme, employing two MAb as molecular thermal switches of polymerase and proof-reading activities.
• Fast PCR: rate of synthesis more than 2x that of Taq DNA polymerase

 

In an internal comparison studies, GB-AMP™ PaCeR™  HP™ was compared in the following aspects with the leading all-round enzymes such as Phusion (Thermo) and eight other leading DNA polymerases for PCR:

• Success rate in amplifying human, mouse, rice, wheat, lambda and plasmid DNA, ranging in size from 305 to 14768 bp
• Sensitivity, i.e., ability to detect low copy templates,
• Speed of amplification with extension time as short as 1 s (for 450 bp) and 5 s (1135 bp)
• Amplification of  High GC template with GC as high as 85%

 

GB-AMP™ PaCeR™  HP™ was clearly the best all-round enzyme for robustness, sensitivity, speed, high GC amplification and Direct PCR!

A tip on the use: The annealing temperature should be chosen based on the Tm values of the primers, in general, 55-65°C. See the user manual for details. 

 

Looking for a similar DNA Polymerase with higher fidelity and amplification speed? Check out our 2× PaCeR (Phanta) Flash Master Mix (P510). 

 

The enzyme generates blunt-ended products and thus products can be cloned by our Blunt End Cloning Kits.

Please note: If you are getting a low yield or even no amplification with PaCeR, please consider lowering the annealing temperature by 3-5 °C and/or increasing the annealing time (doubling). Talk to our Tech Support as you need. 

 

Citations

1. Nash D., et al. Hybrid sequencing reveals the genome of a Chrysochromulina parva virus provides insights into nucleocytoplasmic large DNA virus evolution. BMC Genomics. 2025;26:534.
   doi:10.1186/s12864-025-11700-z
2. Dhaliwal J., Sharma P., et al. Protocol for the efficient and inducible generation of gene knockouts in Candida auris. STAR Protoc. 2024;5(3):102639.
   doi:10.1016/j.xpro.2024.102639
3. Deecker S.R., et al. Type I-F CRISPR-Cas distribution and array dynamics in Legionella pneumophila. G3 (Bethesda). 2020;10(3):1039–1050.
   doi:10.1534/g3.119.400908
4. Hurst J.R., et al. Monkeypox virus shedding despite tecovirimat treatment. J Infect Dis. 2025; advanced online publication.
   doi:10.1093/infdis/jiaf471
5. Monteiro1 VL et al. Ataxin-2 preserves oocyte genome integrity by promoting ribosomal RNA processing. bioRxiv. Posted June 16, 2025.
   doi:10.1101/2025.06.13.659559
6. Roscow O.M.A. Development of a full-length infectious clone of grapevine fanleaf virus and its application to study host-virus interactions. MSc Thesis. University of Guelph; 2019.
   Available at: https://atrium.lib.uoguelph.ca/bitstream/10214/17655/3/Roscow_Olivia_201912_Msc.pdf
7. Goetz J.A., et al. Exploring functional interplay amongst Escherichia coli efflux pumps. Microbiology (Reading). 2022;168(6):001233.
   doi:10.1099/mic.0.001233

Customer Reviews

2x GB-AMP™ PaCeR™ HP™ Master Mix

Our lab has been using 2x GB-AMP™ PaCeR™ HP™ Master Mix for the past few years, and it has consistently delivered excellent results. We rely on this enzyme for a wide range of PCR applications, including cloning.

Works Great

For a long time, I stuck with Q5 or Phusion when doing high-fidelity PCR, but I figured I would give PaCeR a try after hearing a few good things about it. I was very pleased with the results. It amplified larger templates (3-5 kb) quickly and reliably, and the DNA yield was as good or better as other polymerases on the market. The fact that it is less expensive than other polymerases is an extra bonus. Our lab will be using PaCeR going forward!

The best polymerase available in the market so far.

I tested many proprietary and expensive polymerases (Q5, Phusion, Agilent mutagenesis proprietary pFU, and other), but this works the best. Highly recommended for long template ~15kb plasmids and mutagenesis with primers having RES sites and overhangs. In my hand, this is one of the best polymerase available in the market.

The only polymerase we need!

Our lab has been using GB-AMP™ PaCeR™ HP™ DNA polymerase for several years, and we are very satisfied with the results. We use this polymerase for almost every PCR we perform in the lab, including amplification of up to 6kb fragments from plasmids and yeast genomic DNA, and even site-directed mutagenesis. It is the most reliable polymerase that any member of our lab has tried, and we routinely recommend it to our colleagues!

Reliable and affordable polymerase

GB-AMP™ PaCeR™ HP™ DNA polymerase is the most reliable polymerase for bacterial genomic template PCRs. We could not do our recombineering without it! No other polymerase has been able to perform the way PaCeR has for us. We have successfully amplified products as large as 12 kb with minimal optimization.