Zebrafish gABA(Gamma-Aminobutyric Acid) ELISA Kit

SKU: ELK0580

Size:96T

Reactivity:Zebrafish

Alternative Names:4-Aminobutyric Acid

Assay Type:Competitive Inhibition

Sensitivity:16.7 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2.5h

Research Area:Signal transduction;Cardiovascular biology;Neuro science;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish gABA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish gABA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: