Mutagenesis refers to the process of inducing changes in the DNA sequence of an organism. Our mutagenesis kits provide the necessary tools and reagents to introduce specific mutations into a DNA sequence of interest. The three key methods to introduce nucleotide changes into a target gene in an efficient and targeted way include:
1. Site-Directed Mutagenesis:
Designed for the introduction of specific, predetermined mutations into a DNA sequence; these kits often use synthetic oligonucleotides containing the desired mutations and DNA polymerases with proofreading capabilities to incorporate the changes accurately.
2. Random Mutagenesis:
Random mutagenesis kits introduce random nucleotide changes across a target gene typically using error-prone DNA polymerases, such as mutagenic polymerases, to introduce random mutations during DNA amplification.
3. Saturation Mutagenesis:
Saturation mutagenesis aims to generate all possible nucleotide changes at a specific position within a gene. Our kits use a combination of synthetic oligonucleotides, each containing a different nucleotide at the target position, to create a library of mutants.
Our mutagenesis kits enable researchers to generate genetic variants, explore gene function, study drug interactions, explore structure-function relationship of genes, model diseases, direct evolution, perform gene knockout studies, prepare NGS libraries, and engineer proteins for various applications.
