TLR4 ELISA kit (Human)

SKU: SEA753Hu

Size: 96Tests

# of Times Cited in literature: 27

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: TLR4

Target Full Name: Toll Like Receptor 4

Alternative Names: CD284; TOLL; HToll

Target Species: Human

Uniprot: O00206

Gene ID: 7099

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human TLR4 / Toll Like Receptor 4

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.312-20ng/mL

Sensitivity: 0.118ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 4 (TLR4). No significant cross-reactivity or interference between Toll Like Receptor 4 (TLR4) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 4 (TLR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 4 (TLR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 4 (TLR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 4 (TLR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;Pulmonology;

References Citing This Product: Expression of soluble Toll-like receptors in pleural effusions

Amniotic fluid soluble Toll-like receptor 4 in pregnancies complicated by preterm prelabor rupture of the membranes

The association between precancerous gastric lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag A status

Toll-Like Receptor 2 in Serum: a Potential Diagnostic Marker of Prosthetic Joint Infection?

Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination

The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases

Procoagulatory State in Inflammatory Bowel Diseases Is Promoted by Impaired Intestinal Barrier Function

Efficacy of rifaximin on circulating endotoxins and cytokines in patients with nonalcoholic fatty liver disease

Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage.

The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients

НОСИТЕЛЬСТВО ПОЛИМОРФИЗМА Thr399Ile и Asp299Gly ГЕНА TLR4