TDG antibody , Cat. # FNab08570

SKU: FNab08570

Size:100ug

Purification:Immunogen affinity purified

Form:liquid

Purity:?95% as determined by SDS-PAGE

Host:Rabbit

Clonality:polyclonal

Clone ID:

Isotype:IgG

Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)

Background:DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine(5fC) and 5-carboxylcytosine(5caC) in the context of CpG sites and mediates their excision through base-excision repair(BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine(5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil(5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

Immunogen:thymine-DNA glycosylase

Synonyms:

Observed MW:55-60 kDa

Uniprot ID:Q13569

Reactivity:Human, Mouse, Rat

Tested Application:ELISA, WB, IP

Recommended dilution:WB: 1:500-1:2000; IP: 1:200-1:2000

Gene ID:6996

Research Area:Metabolism