Simian C5b-9(Terminal Complement Complex C5b-9) ELISA Kit

SKU: ELK11052

Size:96T

Reactivity:Simian

Alternative Names:MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9

Assay Type:Sandwich

Sensitivity:13.22 ng/mL

Standard:2000 ng/mL

Detection range:31.25-2000 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian C5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian C5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian C5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian C5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

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