Simian BMP4(Bone Morphogenetic Protein 4) ELISA Kit

SKU: ELK11636

Size:96T

Reactivity:Simian

Alternative Names:BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B

Assay Type:Sandwich

Sensitivity:12.2 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Developmental science;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian BMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian BMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian BMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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