Sheep SPB(Surfactant Associated Protein B) ELISA Kit

SKU: ELK8875

Size:96T

Reactivity:Sheep

Alternative Names:SFTPB; PSPB; SFTB3; SFTP3; SP-B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein

Assay Type:Sandwich

Sensitivity:0.56 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;Infection immunity;Pulmonology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep SPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep SPB in the samples is then determined by comparing the OD of the samples to the standard curve.

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