Sheep ALB(Albumin) ELISA Kit

SKU: ELK11435

Size:96T

Reactivity:Sheep

Alternative Names:Serum albumin

Assay Type:Competitive Inhibition

Sensitivity:0.58 μg/mL

Standard:100 μg/mL

Detection range:1.57-100 μg/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep ALB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep ALB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P14639