GeneBio Systems

SEPP1 ELISA kit (Human)

Name: SEPP1 ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEB809Hu
Price: 174900 JPY
Availability: InStock

SKU:SEB809Hu

¥174,900 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 22

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SEPP1

Target Full Name: Selenoprotein P1, Plasma

Alternative Names: SEPP; SeP; SEP-P1; SELP

Target Species: Human

Uniprot: P49908

Gene ID: 6414

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human SEPP1 / Selenoprotein P1, Plasma

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.78-50ng/mL

Sensitivity: 0.31ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selenoprotein P1, Plasma (SEPP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selenoprotein P1, Plasma (SEPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Selenoprotein P1, Plasma (SEPP1). No significant cross-reactivity or interference between Selenoprotein P1, Plasma (SEPP1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selenoprotein P1, Plasma (SEPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selenoprotein P1, Plasma (SEPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selenoprotein P1, Plasma (SEPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selenoprotein P1, Plasma (SEPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Metabolic pathway;Infection immunity;

References Citing This Product: Serum Selenoprotein P Levels in Patients with Type 2 Diabetes and Prediabetes: Implications for Insulin Resistance, Inflammation, and Atherosclerosis

Increased Selenoprotein P Levels in Subjects with Visceral Obesity and Nonalcoholic Fatty Liver Disease

Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemia

Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism

Selenoprotein P is elevated in individuals with obesity, but is not independently associated withinsulin resistance.

Acute Overfeeding Does Not Alter Liver or Adipose Tissue-Derived Cytokines in HealthyHumans.

DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: ashort-term supplementation trial with respect to GPX1 and SEPP1 polymorphism.

Evaluation of the Relationship Between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary Syndrome

Serum selenium and selenoprotein-P levels in autoimmune thyroid dieases patients in a select center a transversal study

Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth

Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury