SAP ELISA kit (Human)

SKU: SEB539Hu

Size: 96Tests

# of Times Cited in literature: 8

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SAP

Target Full Name: Serum Amyloid P Component

Alternative Names: APCS; PTX2; Pentaxin-Related; 9.5S Alpha-1-Glycoprotein

Target Species: Human

Uniprot: P02743

Gene ID: 325

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human SAP / Serum Amyloid P Component

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 1.56-100ng/mL

Sensitivity: 0.65ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Serum Amyloid P Component (SAP). No significant cross-reactivity or interference between Serum Amyloid P Component (SAP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid P Component (SAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Amyloid P Component (SAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Amyloid P Component (SAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid P Component (SAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Infection immunity;

References Citing This Product: Circulation levels of acute phase proteins in patients with Takayasu arteritis

Combined Proteomic and Metabolomic Profiling of Serum Reveals Association of the Complement System with Obesity and Identifies Novel Markers of Body Fat Mass Changes

Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 3-D DIGE

Targeted proteomics predict a sustained complete-response after transarterial chemoembolization and clinical outcomes in patients with hepatocellular carcinoma: a prospective cohort study

Amniotic fluid pentraxins: Potential early markers for identifying intra‐amniotic inflammatory complications in preterm pre‐labor rupture of membranes

Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients