SAA ELISA kit (Human)

SKU: CEA885Hu

Size: 96Tests

# of Times Cited in literature: 9

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SAA

Target Full Name: Serum Amyloid A

Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Target Species: Human

Uniprot: P0DJI8

Gene ID: 6288

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human SAA / Serum Amyloid A

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 39.06-10,000ng/mL

Sensitivity: 14.73ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Serum Amyloid A (SAA). No significant cross-reactivity or interference between Serum Amyloid A (SAA) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Serum Amyloid A (SAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Serum Amyloid A (SAA) and unlabeled Serum Amyloid A (SAA) (Standards or samples) with the pre-coated antibody specific to Serum Amyloid A (SAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Serum Amyloid A (SAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Serum Amyloid A (SAA) in the sample.

Research Area: Infection immunity;Kidney biomarker;Hepatology;

References Citing This Product: Acute Phase Response: Implication in ST-segment Elevation Myocardial Infarction

Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial

No association between vitamin D levels and inflammation markers in patients with acute coronary syndrome

Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study

Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitus

The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome

Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhage

PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME

Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India