Recombinant UCHL1 Monoclonal Antibody

SKU: AN300585P

Size::

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:UCHL1

Target Synonym:UCHL;Uchl1;HEL-117;HEL-S-53;NDGOA;PARK5;PGP 9.5;PGP9.5;PGP95;SPG79;Ubiquitin C-terminal hydrolase L1;Uch-L1

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Rat

Application:WB;IP

Isotype:IgG

Clonality:Monoclonal

Clone NO.:11B6

UNIProt ID:Q00981

Accession:

Background:Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

Concentration:1 mg/mL

Immunogen:Recombinant Rat UCHL1 protein

Buffer:0.2 μm filtered solution in PBS

Purification method:Protein A

Dilution:WB 1:500-1:2000;IP 1-4 μL/mg of lysate

Calculated MW:25 kDa

ObservedMW:25 kDa