Recombinant Topoisomerase I Monoclonal Antibody

SKU: AN301767L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Topoisomerase I

Target Synonym:TOP;TOP1;TOPI

Research Areas:Epigenetics and Nuclear Signaling;Cancer

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IF;FCM;IP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A475

UNIProt ID:P11387

Accession:

Background:DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine.

Concentration:1 mg/mL

Immunogen:Recombinant human Topoisomerase I fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:2000;IHC 1:100-1:500;IF 1:50;FCM 1:50-1:100;IP 1:25-1:50

Calculated MW:91 kDa

ObservedMW:108 kDa