Recombinant Rat SDF-1 Alpha/CXCL12 Alpha protein

SKU: CD00074

Size:2 ug

Background:CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96 % amino acid sequence identity with human CXCL12α.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Stromal-Cell Derived Factor-1 alpha/CXCL12α

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5-1 ng/ml.

Synonyms:SDF-1α/CXCL12α,Sdf1, Rat

Endotoxin:Less than 1 EU/μg of rRtSDF-1α/CXCL12α as determined by LAL method.

Storage:-20ºC

Sequence:KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

uniprot accession:Q9QZD1

Molecular weight:Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 15 mM NaCl.