Recombinant Rat PDGF-AA protein

SKU: CD00082

Size:2 ug

Background:Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).

Research_area:Cardiovascular

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Platelet-derived Growth Factor-AA

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 5. ng/ml, corresponding to a specific activity of > 2. × 15 IU/mg.

Synonyms:PDGF-AA, Rat

Endotoxin:Less than 0.1 EU/μg of rRtPDGF-AA as determined by LAL method.

Storage:-20ºC

Sequence:MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R

uniprot accession:P28576

Molecular weight:Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.