Recombinant Rat IL-33 protein

SKU: CD00104

Size:2 ug

Background:Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a cytokine belonging to the IL-1 superfamily. It is also a proinflammatory protein that may regulate gene transcription and it induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL-33 is constitutively expressed in smooth muscle and airway epithelia and it binds to a high-affinity receptor family member ST2. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Recombinant rat IL-3 contains 156 amino acid residues and it shares 59 % a.a. and 90 % sequence identity with human and Mouse IL-33.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin 33

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse D1S cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 16 IU/mg.

Synonyms:IL-33,IL33,RGD1311155, Rat

Endotoxin:Less than 1 EU/μg of rRtIL-33 as determined by LAL method.

Storage:-20ºC

Sequence:SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM

uniprot accession:Q66H7

Molecular weight:Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM Tris, 3 mM NaCl, pH 8.5.