Recombinant Rat IL-3 Beta protein

SKU: CD00105

Size:5 ug

Background:Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated Mouse mast cells, Mouse keratinocytes and neurons/astrocytes. It exerts its biological activities through binding to Interleukin-3 receptors included α and β subunits, but sequence analysis revealed a number of features indicative of the presence of only one β-subunit in the rat. Interleukin-3 acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Furthermore, rat and human IL-3 share low homology and have not cross species activity.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-3 beta

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse MC-9 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 15 IU/mg.

Synonyms:IL-3β, Rat

Endotoxin:Less than 1 EU/μg of rRtIL-3β as determined by LAL method.

Storage:-20ºC

Sequence:MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC

uniprot accession:P4823

Molecular weight:Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.