Recombinant Rat IL-21 protein

SKU: CD00107

Size:2 ug

Background:Rat IL-21 is produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxic T cells and natural killer cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation with IL-4 of IgG production, and induction of apoptotic effects in na ve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, it promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. Mature rat IL-21 shares 88 % a.a. sequence identity with mouse IL-21.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin 21

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human N1186 cells is less than 5 ng/ml, corresponding to a specific activity of > 2. × 14 IU/mg.

Synonyms:IL-21, IL21,Rat

Endotoxin:Less than 1 EU/μg of rRtIL-21 as determined by LAL method.

Storage:-20ºC

Sequence:HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS

uniprot accession:A3QPB9

Molecular weight:Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.