Recombinant Rat IL-1RA protein

SKU: CD00109

Size:20 ug

Background:IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the co-receptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89 % and 73 % a.a. sequence homology with Mouse and human IL-1RA.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-1 Receptor Antagonist Protein

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by inhibiting IL-1α-dependent proliferation of Mouse D1S cells is less than 15 ng/ml, corresponding to a specific activity of > 6.7 × 13 IU/mg in the presence of 5 pg/ml rRtIL-1α.

Synonyms:IL-1RA, IL1RN,Rat

Endotoxin:Less than 1 EU/μg of rRtIL-1RA as determined by LAL method.

Storage:-20ºC

Sequence:HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ

uniprot accession:P2586

Molecular weight:Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.