Recombinant Rat Fractalkine/CX3CL1 protein

SKU: CD00123

Size:5 ug

Background:CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. In addition, it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Recombinant Rat CX3CL1 contains 76 amino acids and it shares approximately 86 % and 83 % amino acid sequence homology with the Mouse and human protein.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Fractalkine/CX3CL1

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.-1 ng/ml.

Synonyms:Fractalkine/CX3CL1,Cx3c; Scyd1, Rat

Endotoxin:Less than 1 EU/μg of rRtFractalkine/CX3CL1 as determined by LAL method.

Storage:-20ºC

Sequence:QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNG

uniprot accession:O55145

Molecular weight:Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of Rat Fractalkine.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2× PBS, pH 7.4.