Recombinant Rat FGF-21 protein

SKU: CD00126

Size:5 ug

Background:Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and Mouse FGF-21, and it show activity on Mouse and human cells.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Fibroblast Growth Factor-21

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than .5 μg/ml, corresponding to a specific activity of > 2. × 13 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 1 μg/ml of heparin.

Synonyms:FGF-21, Fgf8c,FGF21,Rat

Endotoxin:Less than 1EU/μg of rRtFGF-21 as determined by LAL method.

Storage:-20ºC

Sequence:AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

uniprot accession:Q8VI8

Molecular weight:Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 18 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, 5 mM NaCl, pH 7.4.