Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody

SKU: AN301007L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:Pyruvate Dehydrogenase E1 α

Target Synonym:PDHA1;PDHA;PDHAD;PDHCE1A;PHE1A;E1 alpha polypeptide 1;mitochondrial;ODPA;PDH;PDHE1 A type I;PDHE1-A type I;PDHα;Pyruvate Dehydrogenase (lipoamide) alpha 1;Pyruvate dehydrogenase complex;Pyruvate Dehydrogenase E1 alpha;Pyruvate dehydrogenase E1 component subunit alpha;somatic form

Research Areas:Signal Transduction;Cancer;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Mouse;Rat

Application:WB;IHC

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:1A5

UNIProt ID:P08559

Accession:

Background:The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Concentration:0.2 mg/mL

Immunogen:Recombinant Human Pyruvate Dehydrogenase E1 α protein

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A

Dilution:IHC 1:200-1:1000;WB 1:1000-1:5000

Calculated MW:43 kDa

ObservedMW:43 kDa