Recombinant Pseudomonas aeruginosa Major outer membrane lipoprotein (oprI)

SKU: P11221

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Cardiovascular

Uniprot ID: P11221

Gene Names: oprI

Alternative Name(s): (Outer membrane lipoprotein I)

Abbreviation: Recombinant Pseudomonas aeruginosa oprI protein

Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Source: E.coli

Expression Region: 1-83aa

Protein Length: Full Length

Tag Info: N-terminal 6xHis-GST-tagged

Target Protein Sequence: MNNVLKFSALALAAVLATGCSSHSKETEARLTATEDAAARAQARADEAYRKADEALGAAQKAQQTADEANERALRMLEKASRK

MW: 40.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance:

Reference: "Cloning and analysis of the gene for the major outer membrane lipoprotein from Pseudomonas aeruginosa." Cornelis P., Bouia A., Belarbi A., Guyonvarch A., Kammerer B., Hannaert V., Hubert J.-C. Mol. Microbiol. 3: 421-428(1989)

Function: