Recombinant Proteus mirabilis Serralysin(zapA)

SKU: CSB-EP606126EYY

Size: 200ug. Other sizes are also available. Please Inquire.

In Stock: No

Lead time: 10-20 working days

Research Topic: Microbiology

Uniprot ID: Q11137

Gene Names: zapA

Organism: Proteus mirabilis

AA Sequence: DLLDKSGIFYNYSTKVLPSFDYDTAGKHIAREDSTWNGKYVIGQPAEVTYSFPKWEGKFNQFGNKNPYEFNELQKEHARKSLDAWSDIANIKFTEVAVGNVDGMKASDVKTDITFGNIYDPNGTFQAYATLPNTYAYGKDLSGQAWFSDYHYAGNTTPELGNYGRLTIIHEIGHTLGLMHPGDYNAGQNVPGYLKSDYAEDSRQYTVMSYWDEYETGAHFQGAYAGAPLLHDISAMQYLYGANTTTRTGDDVYGFNSNTGIDYYTATDSNDKLIFSVWDSGGNDTFDFSGFYQDQLIDLRAGNFSDVGGLQKNVSIAQNVTIENAIGGFGNDIIHGNDADNTLIGGEGDDIIYGHSGNNTIYGGRGQDTLHGGTGSNTFIYKEIADSLVTAADKIMDFKTGIDKIDLSTLIQDTFSSKILNFVDNFTGNAGEATLSYNEVTNASELAINAYGYNYNPDFKIDIVGFVNYETDFIV

Expression Region: 17-491aa

Sequence Info: Full Length

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

MW: 68.4 kDa

Alternative Name(s): Extracellular domain metalloprotease

Relevance: One of the virulence factors produced during swarmer cell differentiation of the bacteria, which seems to be associated with pathogenesis. The protease activity is limited to IgA1, IgA2, as well as IgG degradation.

Reference: "Molecular analysis of a metalloprotease from Proteus mirabilis."Wassif C., Cheek D., Belas R.J. Bacteriol. 177:5790-5798(1995)

Purity: Greater than 90% as determined by SDS-PAGE.

Storage Buffer: Tris-based buffer，50% glycerol

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.