Recombinant PRAS40 Monoclonal Antibody

SKU: AN301877L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:PRAS40

Target Synonym:PRAS;AKT1 substrate;AKT1S;AKT1S1;Lobe;PRAS40;AKT1 substrate 1

Research Areas:Cell Biology;Neuroscience;Signal Transduction

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A589

UNIProt ID:Q96B36

Accession:

Background:PRAS40 is a proline-rich protein with a molecular weight of 40 kDa that acts as a substrate for the serine / threonine kinase Akt. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.

Concentration:1 mg/mL

Immunogen:Recombinant human PRAS40 fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:50-1:200

Calculated MW:27 kDa

ObservedMW:40 kDa