Recombinant Pediococcus acidilactici Bacteriocin pediocin PA-1 (pedA)

SKU: P29430

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P29430

Gene Names: pedA

Alternative Name(s): (Pediocin ACH)

Abbreviation: Recombinant Pediococcus acidilactici pedA protein

Organism: Pediococcus acidilactici

Source: E.coli

Expression Region: 19-62aa

Protein Length: Full Length of Mature Protein

Tag Info: N-terminal 6xHis-KSI-tagged

Target Protein Sequence: KYYGNGVTCGKHSCSVDWGKATTCIINNGAMAWATGGHQGNHKC

MW: 20.0 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Bactericidal activity (effective inhibitor of L.monocytogenes).

Reference: "Synthesis, antimicrobial activity and conformational analysis of the class IIa bacteriocin pediocin PA-1 and analogs thereof." Bedard F., Hammami R., Zirah S., Rebuffat S., Fliss I., Biron E. Sci Rep 8: 9029-9029(2018)

Function: