Recombinant Non-Phospho (Active) β-Catenin (Ser33, Ser37, Thr41) Monoclonal Antibody

SKU: AN301881L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Non-Phospho (Active) β-Catenin (Ser33, Ser37, Thr41)

Target Synonym:MRD;PRO;Catenin beta;SW-cl;OK/SW-cl;CTNNB;MRD19;armadillo;beta Catenin;CTNNB1;Beta-catenin;Catenin beta-1;OK;SW-cl.35;OK/SW-cl.35;PRO2286;88kDa;beta 1;Cadherin associated protein;Catenin (cadherin associated protein);Catenin beta 1;CATNB;CHBCAT;CTNB1;DKFZp686D02253;FLJ25606;FLJ37923;OTTHUMP00000162082;OTTHUMP00000165222;OTTHUMP00000165223;OTTHUMP00000209288;OTTHUMP00000209289

Research Areas:Signal Transduction;Neuroscience;Stem Cells;Cancer;Cardiovascular

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IF

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A593

UNIProt ID:P35222

Accession:

Background:β-catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.Non-phospho (Active) β-Catenin (Ser33/37/Thr41) Rabbit mAb specifically recognizes β-catenin that is non-phosphorylated at Ser33, Ser33 or Thr41. Non-phospho β-Catenin (Ser33, Ser33 or Thr41) protein is useful as a readout of stabilized β-catenin protein, and therefore functionally active in cell-cell adhesion and mediating transcriptional activity via the canonical Wnt signaling pathway.

Concentration:1 mg/mL

Immunogen:Recombinant human Non-phospho (Active) β-Catenin (Ser33/37/Thr41) fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:100-1:500;IF 1:50

Calculated MW:85 kDa

ObservedMW:100 kDa