Recombinant Mouse Zinc finger protein 410 (Znf410)

SKU: Q8BKX7

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: Q8BKX7

Gene Names: Znf410

Alternative Name(s): (Another partner for ARF 1)

Abbreviation: Recombinant Mouse Znf410 protein

Organism: Mus musculus (Mouse)

Source: E.coli

Expression Region: 1-478aa

Protein Length: Full Length

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: MLSDELESKPELLVQFVQNTSIPLGQGLVESEAKDITCLSLLPVTEASECSRLMLPDETPNHANSSKEVPSSAVLRSLQVNVGPDGEETRAQTVQKSPEFLTTPESPSLLQDLQPSDSTSFILLNLTRAGLGSSAEHFVFVQDETEDSGADFLSAESTDSSIPWFLRVQELAHDSLIAATRAQLAKNAKTGSNGENVHLGSGDGQPKDSGPLPQAEKKLKCTVEGCDRTFVWPAHFKYHLKTHRNERSFICPAEGCGKSFYVLQRLKVHMRTHNGEKPFMCHESGCGKQFTTAGNLKNHRRIHTGEKPFLCEAQGCGRSFAEYSSLRKHLVVHSGEKPHQCQVCGKTFSQSGSRNVHMRKHHLQLGTTGSQEQDQTAEPLMGSSLLEDASVPNKNLVSMNSQSSLGGESLNLSNTNSILGVDDEVLTERASRPLSSVPDVTHHLVTMQSGRQSYEVSVLTAVNPQELLNQGDLTERQT

MW: 59.7 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Transcription factor that binds to the sequence motif 5'-CATCCCATAATA-3', and is specifically required to silence expression of fetal hemoglobin in adult erythroid cells. Prevents expression of fetal hemoglobin genes HBG1 and HBG2 through CHD4: acts as a direct transcriptional activator of CHD4, a central component of the NuRD complex that represses transcription of fetal hemoglobin genes HBG1 and HBG2 in erythroid cells. May also activate transcription of matrix-remodeling genes such as MMP1 during fibroblast senescence. May activate transcription of the gap junction gene GJC1, perhaps in response to increasing glucose. However, recent studies suggest that ZNF410 is dedicated to regulate expression of a single gene: CHD4.

Reference: "ZNF410 represses fetal globin by singular control of CHD4." Vinjamur D.S., Yao Q., Cole M.A., McGuckin C., Ren C., Zeng J., Hossain M., Luk K., Wolfe S.A., Pinello L., Bauer D.E. Nat. Genet. 53: 719-728(2021)

Function: