Recombinant Mouse Prostatic Acid Phosphatase Protein(Fc Tag)

SKU: PDMM100101

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Prostatic Acid Phosphatase/ACPP

Target Synonym:5'-NT;5'-nucleotidase;Acid phosphatase prostate;ACP 3;ACP3;acpP;Ecto-5'-nucleotidase;PAP;PPAP;Prostatic acid phosphatase;Prostatic acid phosphotase;Thiamine monophosphatase;TMPase

Research Areas:Tags & Cell Markers;Cell Biology;Cancer

Conjugation:

Target Species:Mouse

Expression Host:Mammalian

Application:

Fusion tag:C-Fc

UNIProt ID:Q8CE08

Accession:Q8CE08

Background:ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature Human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, Human ACPP shares 84% aa identity with an Mouse ACPP.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Asn381aa

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:66.8 kDa

ObservedMW:80 kDa