Recombinant Mouse Ngfb Protein(His Tag)

SKU: PDEM100338

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Ngfb

Target Synonym:Beta-nerve growth factor;Beta-NGF;NGF

Research Areas:Neuroscience;Developmental Biology;Cell Biology

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P01139

Accession:P01139

Background:NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor(BDNF), neurotrophin-3(NT-3), and neurotrophin-4(NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta-NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta-NGF and 95.8% with rat beta-NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

Concentration:

Activity:Not validated for activity

Sequence:Ser122-Gly241

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:13.1 kDa

ObservedMW:13 kDa