Recombinant Mouse Interleukin-12/IL-12 Protein

SKU: PKSM041070

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: Interleukin-12;IL-12

Target Synonym: CLMF p35;CLMF p40;Cytotoxic lymphocyte maturation factor 35 kDa subunit;Cytotoxic lymphocyte maturation factor 40 kDa subunit;IL-12;IL-12 subunit p35;IL-12 subunit p40;IL-12A;IL-12B;Interleukin 12;Interleukin-12 subunit alpha;Interleukin-12 subunit beta

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application:

Fusion tag: None

UNIProt ID: P43432;P43431

Accession: P43432&P43431

Background: Mouse IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on murine cells. It is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.

Concentration:

Activity: Not validated for activity

Sequence: Met23-Ser335&Arg23-Ala215

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 35.8&21.7 kDa

ObservedMW: 40-55&20-28 kDa