Recombinant Mouse INS protein (GST tag)

SKU: PDEM100243

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:INS

Target Synonym:IDDM;IRDN;Ins-2;Insulin B chain;Proinsulin;ins

Research Areas:Signal Transduction;Neuroscience;Cardiovascular;Metabolism

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-GST

UNIProt ID:P01326

Accession:P01326

Background:Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Concentration:

Activity:Not validated for activity

Sequence:Phe 25-Asn 110

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:34.4 kDa

ObservedMW:30 kDa