Recombinant Mouse IL-5RA/IL-5 Rα Protein (Human Cells, His Tag)

SKU: PKSM041096

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL-5RA;IL-5 Rα

Target Synonym: CD125;IL-5 receptor subunit alpha;IL-5R subunit alpha;IL-5RA;IL-5R-alpha;Interleukin-5 receptor subunit alpha;Il5r;Il5ra; CDw125;IL-5RαIl5r

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P21183

Accession: P21183

Background: Interleukin 5 Receptor alpha (IL-5 Rα), also known as CD125, is a hematopoietin receptor that plays a dominant role in eosinophil biology. Mature mouse IL-5 Rα consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain. The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL-5 Rα and the transmembrane common β chain (βc/CD131) which is shared with the receptor complexes for IL-3 and GM-CSF. IL-5 Rα binds IL-5 at low affinity and then associates with preformed βc oligomers to form the signaling-competent receptor complex. IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the up-regulation of transmembrane IL-5 Rα followed by eosinophilic differentiation and activation. IL-5 Rα also promotes the differentiation of basophils and B cells. Exposure of mature eosinophils to IL-5 attenuates their IL-5 responsiveness by inducing the down-regulation of surface IL-5 Rα and increased production of soluble IL-5 Rα.

Concentration:

Activity: Not validated for activity

Sequence: Asp18-His339

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 37.6 kDa

ObservedMW: 48 kDa