Recombinant Mouse IL-1RA/IL1RN Protein

SKU: PKSM041046

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL-1RA;IL1RN

Target Synonym: IL1F;MVCD;IL-1ra;F630041P17Rik;DIRA;ICIL-1RA;IL1 inhibitor;IL1F3;IL1RA;IL-1ra3;IL-1RN;Interleukin-1 Receptor Antagonist Protein;IRAP;MVCD4;Il1rn;F630041P17Rik

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P25085

Accession: P25085

Background: Interleukin-1 receptor antagonist protein (Il1rn), also known as IL-1ra, IRAP or IL1 inhibitor, is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1 alpha (IL1A) and interleukin 1 beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. The mouse Il1rn gene encodes a 178 amino acids (aa) protein with a 26 aa signal peptide. Mouse Il1rn protein shares 26% and 19% identity with its homologues IL-1 beta and IL-1 alpha, respectively. Il1rn can Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling, but has no interleukin-1 like activity. Recently, an recombinant human Il1rn protein is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.

Concentration:

Activity: Measure by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 cells.The ED₅₀ for this effect is <50 ng/mL.

Sequence: Arg27-Gln178

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.3 kDa

ObservedMW: 17 kDa