Recombinant Mouse IL-1A/IL-1α Protein

SKU: PKSM041080

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-1A;IL-1α

Target Synonym: Hematopoietin;IL1F;Hematopoietin-1;IL1;IL1-A;IL1F1;IL-1α;Preinterleukin 1 Alpha;Pro-Interleukin-1-Alpha;Il-1a;Interleukin-1 alpha;IL-1 alpha;Il1a

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P01582

Accession: P01582

Background: Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

Concentration:

Activity: Measure by its ability to induce D10.G4.1 cells proliferation.The ED₅₀ for this effect is <5 pg/mL.The specific activity of recombinant mouse IL-1 alpha is > 2 x 10⁸ IU/mg.

Sequence: Ser115-Ser270

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.9 kDa

ObservedMW: 20 kDa