Recombinant Mouse IL-10 protein

SKU: CD00393

Size:2 ug

Background:Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2). The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Rα/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature Mouse IL-10 shares 70 %-77 % amino acid sequence identity with human, bovine, porcine IL-10, but Mouse IL-10 does not act on human cells.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Interleukin-10

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:IL-1,CSIF, IL-, If2a, Il1, Mouse

Endotoxin:Less than 1 EU/μg of rMuIL-10 as determined by LAL method.

Storage:-20ºC

Sequence:SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS

uniprot accession:P18893

Molecular weight:Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 16 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.