Recombinant Mouse IFN- Lambda3/IL-28B protein

SKU: CD00407

Size:5 ug

Background:Mouse IFN-lambda 2 (IL-28A), IFN-lambda 3 (IL-28B) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid sequence identity) and type I IFN family (15 - 19% a.a. sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28B shares 63%, 63%, and 52% a.a. identity with human IL-28A, IL-28B, and IL-29, respectively.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Interferon-lambda 3/Interleukin-28B

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 14 IU/mg.

Synonyms:IFN-λ3/IL-28B,Il; IFL; Il2; Il28; IFL-1; INF-l; Il28b; IL-28B; If1ia2; INF-alpha; INF-lambda,Ifnl3, Mouse

Endotoxin:Less than 1 EU/μg of rMuIFN-λ3/IL-28B as determined by LAL method.

Storage:-20ºC

Sequence:DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV

uniprot accession:Q8CGK6

Molecular weight:Approximately 19.6 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.