Recombinant Mouse IFN- Gamma protein

SKU: CD00410

Size:20 ug

Background:Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with rat IFN-γ (86 %). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Interferon-gamma

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by an anti-viral assay using Mouse L929 cells infected with encephalomyocarditis (EMC) virus is less than .8 ng/ml, corresponding to a specific activity of > 1.3 × 16 IU/mg.

Synonyms:IFN-γ, IFN-g, If, If2f, Ifg,IFNG,Mouse

Endotoxin:Less than 1 EU/μg of rMuIFN-γ as determined by LAL method.

Storage:-20ºC

Sequence:HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC

uniprot accession:P158

Molecular weight:Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4, containing 5 % trehalose.