Recombinant Mouse Carbonic Anhydrase VIII/CA8 Protein (His Tag)

SKU: PKSM040495

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: AW546993;Ca8;Cals;Cals1;Car8;Carp;RP23-180H12.1;wdl

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Enzyme

Fusion tag: C-His

UNIProt ID: P28651

Accession: P28651

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

Concentration:

Activity: Measured by its esterase activity. The specific activity is > 5 pmoles/min/μg.

Sequence: Met 1-Gln 291

Purity: > 88 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 34.5 kDa

ObservedMW: 37 kDa