Recombinant Mouse Activin Receptor 2B/ACVR2B Protein (His Tag)

SKU: PKSM040825

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: 4930516B21Rik;ActRIIB

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application: Cell culture;ELISA

Fusion tag: C-His

UNIProt ID: P27040

Accession: NP_031423.1

Background: ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.

Concentration:

Activity: 1. Immobilized human ACVR2B at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is 0.161 μg/mL. 2. Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 μg/mL. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-0.8 µg/mL in the presence of 10 ng/mL recombinant Activin A.

Sequence: Met 1-Thr 134

Purity: > 94 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 14.8 kDa

ObservedMW: 33-37 kDa