Recombinant Modification methylase MunI(munIM)

CSB-EP331901MLZ

Size: 200ug. Other sizes are also available. Please Inquire.

In Stock: No

Lead time: 10-20 working days

Research Topic: Others

Uniprot ID: P43641

Gene Names: munIM

Organism: Mycoplasma sp.

AA Sequence: MENKVTAYSIYNKKAKKNTKVNPLDEVFPQLPRKKYQVIYADPPWDYGGKMQYDKSTIKSENEGFKRDIFISSASFKYPTLKLKELQQLDVPSITADDCILFMWTTGPQMANSILLGESWGFEYKTVAFVWDKMVHNPGRYTLSQTEFVLVFKKGKIPTPRGARNVRQLLQIHRGQHSEKPYAVIDGITKMFPALDKIELFARNNFVGWDNWGLEIPDNKIEIPTQGEIDENK

Expression Region: 1-233aa

Sequence Info: Full Length

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

MW: 42.9 kDa

Alternative Name(s): Adenine-specific methyltransferase MunI ;M.MunI

Relevance: This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease.

Reference: CAATTG-specific restriction-modification munI genes from Mycoplasma sequence similarities between R.MunI and R.EcoRI.Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A.Gene 142:1-8(1994)

Purity: Greater than 90% as determined by SDS-PAGE.

Storage Buffer: Tris-based buffer，50% glycerol

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.