Recombinant Human Zic3 protein (His tag)

SKU: PDEH100975

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Zic3

Target Synonym:ZIC3;Zinc finger protein 203;Zinc finger protein ZIC 3;Zinc finger protein of the cerebellum 3

Research Areas:Developmental Biology;Epigenetics and Nuclear signalin;Stem Cells

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-His & C-His

UNIProt ID:O60481

Accession:O60481

Background:ZIC3, or zic family member 3, is a molecule that regulates early embryonic patterning in vertebrates. ZIC3 has putative roles in a number of developmental signalling pathways that have distinct roles in establishing the left-right axis. In addition, ZIC3 is also considered as target of MiR-564, a tumor suppressor in human lung cancer. Variants in the ZIC3 gene are rare, but have demonstrated their profound clinical significance in X-linked heterotaxy, affecting in particular male patients with abnormal arrangement of thoracic and visceral organs.

Concentration:

Activity:Not validated for activity

Sequence:Met 1-Val467

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:51.3 kDa

ObservedMW:52 kDa