Recombinant Human Voltage-dependent anion-selective channel protein 1 (VDAC1), partial

SKU: P21796

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Cancer

Uniprot ID: P21796

Gene Names: VDAC1

Alternative Name(s): Outer mitochondrial membrane protein porin 1 Plasmalemmal porin Porin 31HL Porin 31HM

Abbreviation: Recombinant Human VDAC1 protein, partial

Organism: Homo sapiens (Human)

Source: E.coli

Expression Region: 2-25aa

Protein Length: Partial

Tag Info: N-terminal GST-tagged

Target Protein Sequence: AVPPTYADLGKSARDVFTKGYGFG

MW: 29.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed: 11845315, PubMed: 18755977, PubMed: 20230784, PubMed: 8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed: 15033708, PubMed: 25296756).

Reference: "Characterization of the human porin isoform 1 (HVDAC1) gene by amplification on the whole human genome: a tool for porin deficiency analysis." Messina A., Guarino F., Oliva M., van den Heuvel L.P., Smeitink J., De Pinto V. Biochem. Biophys. Res. Commun. 270: 787-792(2000)

Function: