Recombinant Human Uteroglobin/SCGB1A1 Protein (His Tag)

SKU: PKSH031418

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CC10;CCPBP;CCSP;Clara cell phospholipid-binding protein;Clara cells 10 kDa secretory protein;SCGB1A1;Secretoglobin family 1A member 1;UGB;UP-1;UP1;Urinary protein 1;Urine protein 1;Uteroglobin

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P11684

Accession: P11684

Background: Uteroglobin (UG); also known as Secretoglobin 1A member 1 (SCGB1A1); Blastokinin; Clara cell secretor protein (CCSP) or Clara cell-specific 10-kDa protein (CC10); is the founding member of the secretoglobin family of small; secreted; disulfide-bridged dimeric proteins found only in mammals. This protein is mainly expressed in lung; with anti-inflammatory/immunomodulatory properties. Previous in vitro studies demonstrated that CCAAT/enhancer-binding proteins (C/EBPs) are the major transcription factors for the regulation of SCGB1A1 gene expression; whereas FOXA1 had a minimum effect on the transcription. Uteroglobin is a multifunctional protein with antiinflammatory/immunomodulatory properties. Uteroglobin inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone; retinols; polychlorinated biphenyls; phospholipids; and prostaglandins. In addition to its antiinflammatory activities; Uteroglobin manifests antichemotactic; antiallergic; antitumorigenic; and embryonic growth-stimulatory activities. The tissue-specific expression of the Uteroglobin gene is regulated by several steroid hormones; although a nonsteroid hormone; prolactin; further augments its expression in the uterus. Based on its anti-inflammatory and antiallergic properties; Uteroglobin is a potential drug target. The mechanism of Uteroglobin action is likely to be even more complex as it also functions via a putative receptor-mediated pathway.

Concentration:

Activity: Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cell line. When 5 x 10⁴cells/well are added to human SCGB1A1 coated plates (2 μg/ml and 100 μl/well), approximately > 30% will adhere after one hour at 37 ℃.

Sequence: Met 1-Asn 91

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 9.2 kDa

ObservedMW: 9.2 kDa