Recombinant Human UBE2C protein (His Tag)

SKU: CD00729

Size:10 ug

Background:Ubiquitin Conjugating Enzyme E2 C belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2C gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2C catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis. This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions.

Research_area:Others

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Ubiquitin Conjugating Enzyme E2 C, His

Biological activity:Data is not available.

Synonyms:UBE2C, UBCH1, dJ447F3.2, Human

Endotoxin:Less than 1 EU/μg of rHuUBE2C, His as determined by LAL method.

Storage:-20ºC

Sequence:MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P

uniprot accession:O762

Molecular weight:Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids (a.a.) of human UBE2C/UBCH1 and 12 a.a. vector sequence including 6 × His tag at N-terminus.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.