Recombinant Human Transmembrane 4 L6 family member 1 (TM4SF1)-VLPs (Active)

SKU: P30408

Size: 100ug. Other sizes are also available.

Activity: Yes

Research Areas: Cancer

Uniprot ID: P30408

Gene Names: TM4SF1

Alternative Name(s): M3S1;TAAL6

Abbreviation: Recombinant Human TM4SF1 protein-VLPs (Active)

Organism: Homo sapiens (Human)

Source: Mammalian cell

Expression Region: 1-202aa

Protein Length: Full Length

Tag Info: C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Target Protein Sequence: MCYGKCARCIGHSLVGLALLCIAANILLYFPNGETKYASENHLSRFVWFFSGIVGGGLLMLLPAFVFIGLEQDDCCGCCGHENCGKRCAMLSSVLAALIGIAGSGYCVIVAALGLAEGPLCLDSLGQWNYTFASTEGQYLLDTSTWSECTEPKHIVEWNVSLFSILLALGGIEFILCLIQVINGVLGGICGFCCSHQQQYDC

MW: 23 kDa

Purity: The purity information is not available for VLPs proteins.

Endotoxin: Less than 1.0 EU/μg as determined by LAL method.

Biological_Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TM4SF1 at 5 μg/mL can bind Anti-TM4SF1 recombinant antibody(CSB-RA023615MA1HU). The EC50 is 4.079-4.472 ng/mL. The VLPs (CSB-MP3838) is negative control.

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Relevance:

Reference: "Cloning and expression of the tumor-associated antigen L6." Marken J.S., Schieven G.L., Hellstroem I., Hellstroem K.E., Aruffo A. Proc. Natl. Acad. Sci. U.S.A. 89: 3503-3507 (1992) "Membrane topology of the L6 antigen and identification of the protein epitope recognized by the L6 monoclonal antibody." Marken J.S., Bajorath J., Edwards C.P., Farr A.G., Schieven G.L., Hellstroem I., Hellstroem K.E., Aruffo A. J. Biol. Chem. 269: 7397-7401 (1994)

Function: