Recombinant Human TNFRSF14 Protein(hIgG1 Fc Tag)

SKU: PDMH100449

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Immune Checkpoint Proteins

Abbreviation:TNFRSF14

Target Synonym:UNQ;PRO;Tumor Necrosis Factor Receptor-Like;Tumor Necrosis Factor Receptor Superfamily Member;Tnfrsf;CD270;Herpes Virus Entry Mediator A;Herpesvirus Entry Mediator A;TR2;Tumor Necrosis Factor Receptor Superfamily Member 14;Tumor Necrosis Factor Receptor-Like 2;HVEA;HVEM;TNFRSF14;UNQ329;PRO509

Research Areas:Immunology;Signal Transduction;Cancer;Microbiology

Conjugation:

Target Species:Human

Expression Host:Mammalian

Application:

Fusion tag:C-hIgG1 Fc

UNIProt ID:Q92956-1

Accession:Q92956-1

Background:Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.

Concentration:

Activity:Not validated for activity

Sequence:Leu39-Val202

Purity:> 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method

Calculated MW:42.9 kDa

ObservedMW:50-55 kDa